Different results on Diamond

CHUAI

New member
Hi, I have a question. I aligned sequences (attached files) using Diamond version 0.9.24, I found no sequences aligned. then I compared to the result output in Oct. last year( sorry I forget its version), but I found a sequence ever aligned.

In order to which result is reliable, I verified them using blast+ version 2.9.0, I found the result was same as the oldest diamond result though the same evalue 1e-5 used.

I am very confused, could you tell me how to solve this problem if you know, thanks a lot!!!

Happy new year.
 

CHUAI

New member
[oldest version Diamond result]

H2.bin.75.faGL000721 YP_005796289.1 31.6 405 244 4 4 1140 4 401 5.9e-59 217.2
H2.bin.75.faGL000044 YP_005796289.1 24.2 491 285 5 1 1470 1 405 1.5e-38 149.8

[Blast+ version 2.9.0]
H2.bin.75.faGL000721 YP_005796289.1 31.605 405 244 4 4 1140 4 401 9.87e-75 231
H2.bin.75.faGL000044 YP_005796289.1 32.328 232 154 2 778 1470 176 405 2.73e-38 137
H2.bin.75.faGL000044 YP_005796289.1 38.356 73 45 0 1 219 1 73 3.95e-13 63.2

[newest version Diamond result:]
nothing

THANKS!!!
 

Attachments

Benjamin Buchfink

Administrator
Staff member
I do find the H2.bin.75.faGL000044 hit when using the latest version of Diamond in sensitive mode. The other query is not included in your file. Maybe this is due to the more aggressive repeat masking in earlier versions. But maybe you also ran Diamond in sensitive mode back then but not now?
 
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