query fasta format issues

carlauranga

New member
Hi, my sequences are in the following format, numbered consecutively. I am not able to match any of these peptides to the entire Uniref90.dmnd database. Could you please help me? Thank you in advance.

>1
LLLARKLKLNLLLLLVLAAK
>2
DEARRRMRRWWNARHNHPRRRLWVSRMPR
>3
HDMCKWGEWWYQYLPTAYYDCMMAK
>4
VKLLKKKKKGAKLLVSVCK
>5
DEGVRRLCSHK
>6
CCMMMGCCNDCDYCDCK
>7
SGEEGNFMSEMENK
>8
SCGMCHVMEESQSK
>9
NMGDCCCCEEMMMNK
>10
LLLTLKVKLLLVREKKTK
>11
LGKLKKLLPSWLPKKGVKKGK
>12
DDDCMNCMSGAQPGK
>13
KVPRVDAHKKKVDVLTEFVPPGK
 

Benjamin Buchfink

Administrator
Staff member
Try to use the options --more-sensitive --masking 0 --comp-based-stats 0 -e 100. Still, Diamond is not ideal for mapping such short sequences.
 
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